Method for inducing differentiation of pluripotent stem cells into germline stem cell-like cells

摘要

The present invention is a method for producing a sperm stem cell-like cell in vitro from an isolated pluripotent stem cell-derived primordial germ cell-like cell, comprising the steps of: Co-culturing in culture to obtain a reconstituted testis, and (2) culturing the reconstituted testis obtained by gas-liquid interface culture to induce DDX4-positive and PLZF-positive cells in the reconstituted testis And a method; and a method for producing a germ line stem cell-like cell, comprising dissociating a sperm stem cell-like cell obtained by the method from a reconstituted testis, and culturing the sperm stem cell under conditions capable of inducing a germ line stem cell. provide.