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METHOD FOR INDUCING DIFFERENTIATION OF PLURIPOTENT STEM CELLS INTO GERMLINE STEM CELL-LIKE CELLS
METHOD FOR INDUCING DIFFERENTIATION OF PLURIPOTENT STEM CELLS INTO GERMLINE STEM CELL-LIKE CELLS
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机译:诱导多能干细胞分化为生殖干细胞样细胞的方法
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摘要
Provided are: a method for in vitro producing spermatogenic stem cell-like cells from primordial germ cell-like cells derived from isolated pluripotent stem cells, said method comprising (1) a step for co-culturing the primordial germ cell-like cells with gonad somatic cells by suspension culture to give a reconstituted testis, and (2) a step for culturing the reconstituted testis by gas/liquid interfacial culture so as to induce DDX4-positive and PLZF-positive cells in the reconstituted testis; and a method for producing germline stem cell-like cells, said method comprising dissociating the spermatogenic stem cell-like cells obtained by the aforesaid method from the reconstituted testis and culturing the same under such conditions that germline stem cells can be induced from spermatogenic stem cells.
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