NUCLEIC ACID ENZYME-MEDIATED SIGNAL AMPLIFICATION FOR BIOSENSING

摘要

Described is an approach that takes advantage of rolling circle amplification (RCA) and an RNA-cleaving DNAzyme (RCD) to achieve massive signal amplification for biosensing via a cross-feedback mechanism. An RCA reaction generates copies of an RCD that triggers a reaction cascade designed to generate additional DNA assemblies for RCA. These cross-feedback actions work autonomously to turn limited molecular recognition events into massive amounts of DNA amplicons that can be conveniently detected. This approach was demonstrated for biosensing of a microRNA sequence and a bacterium.