HIGH-THROUGHPUT AND SINGLE NUCLEOTIDE RESOLUTION TECHNIQUES FOR THE DETERMINATION OF RNA POST-TRANSCRIPTIONAL MODIFICATIONS

摘要

Disclosed herein are methods and associated compositions and kits for identifying nucleoside modifications. Particularly exemplified herein are methods that involve inducing base misincorporation at locations in an RNA that harbor a nucleoside modification. The resulting cDNA can then be subjected to next generation sequencing to identify mutations with respect to a reference sequence. Moreover, the type of nucleoside modification can be determined based on the substitution pattern of a given nucleoside modification.