SINGLE-GENE SINGLE-BASE RESOLUTION RATIO DETECTION METHOD FOR RNA CHEMICAL MODIFICATION

摘要

Provided is a method for detecting the chemical modification of a target RNA site X, comprising the steps as follows: (1)acquiring an RNA sample and selecting in the RNA sample a target RNA segment comprising the target RNA site X; (2)SELECT; (3)PCR amplification; (4)comparing the PCR cycle threshold value with a reference PCR cycle threshold value, or comparing the PCR amplification product quantity with a reference PCR amplification product quantity, so as to determine whether there is a target chemical modification in the target RNA site X. Further provided are a method for identifying a substrate target site of RNA modification enzyme or RNA demodification enzyme and a method for quantifying an RNA modification rate in a transcript.