METHOD AND KIT FOR DETECTING RNA N6-METHYLADENINE MODIFICATION AT SINGLE-BASE RESOLUTION IN RANGE OF WHOLE TRANSCRIPTOME

摘要

Disclosed is a method and kit for detecting an RNA N⁶-methyladenine modification at a single-base resolution in the range of a whole transcriptome. According to the method, on the basis of an N⁶-allyl label of in-vivo ribonucleic acid (RNA) adenine and a chemical treatment, base mutation of the in-vivo RNA adenine in the process of reverse transcription into DNA is induced, and a mutation site is then recognized by means of nucleic acid sequencing, so that an a⁶A site is obtained, wherein the site is a site originally modified by m⁶A in cell RNA. By means of the method, the specific label of N⁶-allyladenine in a cell is achieved for the first time, and the label can not only be used for replacing an N⁶-methyladine site in the cell, but also can be positioned by means of mutation sequencing.