METHOD FOR IDENTIFYING MITOCHONDRIAL DNA IN EXTRACELLULAR VESICLES AND TREATMENT OF MTDNA-RELATED DISORDERS AND CANCER

摘要

Isolation of large quantities of mtDNA from cancer patient circulating microvesicles and exosomes as well as cell lines including fibroblasts and bone marrow stromal cells provides a useful biological tool -microvesicles derived mtDNA- to quantify mtDNA, diagnose and study mitochondria disorders and transfer molecules of mtDNA in recipient cells (mtDNA genetic therapy). The treatment of hormonal therapy derived dormant cancer cells with CAF-derived mtDNA^hi EVs promotes an escape from metabolic quiescence and hormonal therapy resistant disease both in vitro and in vivo.