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Compounds and methods for CRISPR/Cas-based genome editing by homologous recombination
Compounds and methods for CRISPR/Cas-based genome editing by homologous recombination
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机译:通过同源重组进行基于CRISPR / Cas的基因组编辑的化合物和方法
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摘要
The present invention relates to guide RNAs comprising adaptor segments having one or more modifications, and their use in homologous recombination by CRISPR:Cas systems. The modified adaptor segments are resistant to degradation by RNaseH. The present invention also relates to a dual guide RNA strategy in which a first guide RNA directs a Cas enzyme to make a double-strand break at a first target sequence, and a second guide RNA comprises an adaptor segment attached to a donor polynucleotide, and binds a second target sequence that is offset from the first target sequence.
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