PROCESS OF OBTAINING POLYMERIC NANOPARTICLES LOADED WITH L-ASPARAGINASE AND NANOPARTICLES OBTAINED

摘要

The present invention patent (PI) concerns a process of obtaining nanoparticles encapsulating L-asparaginase and evaluation of the enzymatic activity in the degradation of L-asparagine, after encapsulation. Thus, said process can be categorized as a pharmaceutical and / or pharmacologically active product considering that it is a process of obtaining polymeric nanoparticles using chitosan and alginate as polymers, and, therefore, it has direct application in the areas of pharmacy, medicine, biomedicine, chemistry and biotechnology. In addition, this invention describes the encapsulation of the L-asparaginase enzyme in nanoparticles for preferential treatment of leukemia. For the development of said process of obtaining polymeric nanoparticles using cross-linked chitosan with sodium tripolyphosphate using the ionotropic gelation and alginate coating method, as well as the nanoparticles obtained, the following steps 1, 2 and 3 should be considered: (1) preparation of the chitosan solution and sodium tripolyphosphate separately, (2) ionotropic gelation, (3) alginate coating. Finally, it is evident that the nanoencapsulation of L-asparaginase with suitable polymers aims at a slow release of the active substance, an increase in the enzymatic activity of the encapsulated form, and, consequently, increases its bioavailability and therapeutic effect. In addition, the final product can be an active ingredient for new pharmaceutical formulations.