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Method for identifying anti-Toxocara IgG marker in blood serum

机译:血清中抗弓形虫IgG标记物的鉴定方法

摘要

The invention relates to medicine, namely to a method for identifying anti-Toxocara IgG marker in blood serum and can be used for diagnosing human toxocariasis.Summary of the invention consists in the study of blood serum in an immunoenzyme test using a microplate with adsorbed Ag toxocara canis and determining the optical density values of the samples by the photometric method at a wavelength of 450 nm, then determining the ratio of the average optical density of the patient's serum x 10 and the average optical density of the negative control samples, and if the ratio is up to 9 NTU, it is considered that the result is negative, if it is greater than 11 NTU, the result is positive, and samples with the result of 9…11 NTU are treated with 25% kaolin suspension, then is repeated the immunoenzyme test with subsequent determination of the indicated ratio to determine the negative result if the ratio is up to 9 NTU and positive if it is greater than 11 NTU.
机译:本发明涉及药物,即一种在血清中鉴定抗弓形虫IgG标志物的方法,可用于诊断人的弓形虫病。本发明的概述在于用吸附有Ag的微孔板在免疫酶试验中研究血清。犬弓形虫并通过光度法在450 nm波长处确定样品的光密度值,然后确定患者血清的平均光密度x 10与阴性对照样品的平均光密度之比,如果该比率最高为9 NTU,则认为结果为负,如果大于11 NTU,则结果为正,将结果为9…11 NTU的样品用25%高岭土悬浮液处理,则为重复免疫酶试验,随后确定指示的比例,如果比例最高为9 NTU,则确定阴性结果,如果比例大于11 NTU,则为阳性结果。

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