Flow microsystem for a layered culture of normal and cancer cells

摘要

The flow microsystem for the layer-based production of normal cancer cells consists of three plates: hydrophilic bottom glass plate (3),a hydrophobic central plate (2) of polymer, equipped with six circular cavities (7) and a hydrophobic top plate (1) of polymer, equipped with two linear microchannels (1a) with three microholes (4) each in a shape close to the oval. Depth openings (7) are located in a place corresponding to the centre of the microholes (4) in the upper plate (1),In addition, the microchannels (1a) at the entrance are equipped with entry holes (5) and at the exit, the microchannels (1a) connect and end with the exit hole (6). Plates (1, 2 and 3) are permanently combined using an oxygen plasma generator and the housing system (4) in the microsystem is linear, bi-functional and consistent with the housing system on the standard Sarstedt 5022411 multi-column plate.