Flow microsystem evaluating the effectiveness of cancer therapy procedures taking into account the size of spheroids and the presence of normal cells

摘要

The flow-through microsystem for assessing the effectiveness of cancer treatment procedures, taking into account spheroid size and the presence of normal cells, consists of the bottom plate (1),and top plate (2),of poly(dimethylsiloxane) and these plates are permanently combined with oxygen plasma. The bottom plate (1) contains two separate structures and each structure has a main flow microchannel (3),running evenly over two secondary microchannels (4),one of which contains four flat microchambers (5),Whereas the second contains four microchambers, the first two (6) having seven small breeding microholes and the second two (7) having seven large breeding microholes. Micro-structures include input channels (8),Corresponding channels (9) and four inlet and outlet channels (10) and additionally one of the structures contains four semi-circular microchannels in its structure linking the breeding chambers, two larger (11) and two narrower (12) and the top plate (2) is a flat plate with eight holes: two medium-changing openings (8a),two return holes (9a) and four entry-exit holes (10a).