Synthesis and Apoptotic Efficacy of Biosynthesized Silver Nanoparticles UsingAcacia lucianaFlower Extract in MCF-7 Breast Cancer Cells: Activation of Bak1 and Bclx for Cancer Therapy

摘要

Due to unique properties of green silver nanoparticles, especially chemical and biological properties, they are one of the most used metallic nanoparticles in modern healthcare applications. Accordingly, we synthesized Ag-NPs usingAcacia lucianaas a reducing and stabilizing agent, to elucidate its apoptosis inducing ability in MCF-7 cancer cells. The Ag-NPs were characterized using FTIR and FESEM. These instruments confirmed that biosynthesized nanoparticles are rod-shaped with average size of 50 nm and negative surface charge of 2.59 mV. The in vitro anticancer effects using MTT assay revealed that the Ag-NPs showed inhibition of cell survival in a time and dose-dependent manner with IC(50)value of 4.37 mg/mL. Also, the expression of pro- and antiapoptotic genes was measured using real-time PCR. The results hinted that Ag-NPs can significantly increase the Bak1/Bclx ratios through the change in mitochondrial membrane permeability and be used as a therapeutic agent breast cancer cells.