BIONIC INTESTINAL ORGAN-ON-A-CHIP, AND PREPARATION METHOD THEREFOR AND APPLICATION THEREOF

摘要

Disclosed are a bionic intestinal organ-on-a-chip, and a preparation method therefor and application thereof. The bionic intestinal organ-on-a-chip has a fluid passage; a porous membrane is provided in the fluid passage; the porous membrane separates the fluid passage into an upper fluid passage and a lower fluid passage; several projections for simulating the intestinal villus structure and several through holes for simulating the intestinal absorption function are distributed on the porous membrane. The upper fluid passage is used for culturing intestinal cells, and the lower fluid passage is used for collecting metabolites; the projections on the porous membrane are used for simulating the intestinal villus structure, and the through holes are used for simulating the intestinal absorption function. Since a three-dimensional stent structure of the several projections is distributed on the porous membrane in the chip, the difference in intestinal tissue structures generated among different batches of chips can be reduced greatly, and the reproducibility is good. The present bionic intestinal organ-on-a-chip can dynamically culture intestinal cells by using perfusion so as to simulate the dynamic intestinal microenvironment, and is applicable to researches in intestinal diseases, drug screening, food safety, and the like.