BIONIC INTESTINAL-HEPATIC ORGAN CHIP, PREPARATION METHOD THEREFOR AND APPLICATION THEREOF

摘要

A microfluidics-based bionic intestinal-hepatic organ chip, a preparation method therefor and an application thereof. The bionic intestinal-hepatic organ chip has a fluid passage; a porous membrane (130) is provided in the fluid passage; the fluid passage is divided into an upper fluid passage (111) and a lower fluid passage (121) by the porous membrane (130); a plurality of groove structures (122) is provided at the bottom of the lower fluid passage (121). Intestinal cells are inoculated in the upper fluid passage (111), and intestinal epithelial tissues having metabolism and absorption functions are formed on the porous membrane (130). Hepatic cells are inoculated in the lower fluid passage (121); due to the fact that the plurality of groove structures (122) is provided at the bottom of the lower fluid passage (121), after the hepatic cells are inoculated, the hepatic cells are gathered in the groove structures (122) to form hepatic cell balls. The three-dimensional dynamically cultivated hepatic cells are more similar to human liver cells and have a better liver function. Use of the chip can simulate a circulation system of intestine and liver more accurately, and is beneficial to research in the fields of pharmacokinetics, drug screening, and food safety.