METHOD OF ATTACHING ADAPTORS TO SINGLE-STRANDED REGIONS OF DOUBLE-STRANDED POLYNUCLEOTIDES

摘要

A method of attaching an adapter to a polynucleotide comprising: providing a double stranded polynucleotide comprising a single stranded break point within its polynucleotide sequence; contacting said double stranded polynucleotide with an exonuclease to form a single stranded region initiated at said break point; attaching an adapter to said single stranded region.