METHODS AND COMPOSITIONS FOR HOMOLOGY-DIRECTED REPAIR OF CAS ENDONUCLEASE MEDIATED DOUBLE STRAND BREAKS

摘要

Compositions and methods are provided for effecting homology-directed repair at the site of a double-strand-break repair in a polynucleotide. In some aspects, the frequency of HDR is improved as compared to a control method. In some aspects, the ratio of HR to NHEJ is increased. In some aspects, the percentage of HR relative to the total number of mutant reads is increased.