METHOD FOR EVALUATING QUALITY OF DEPHOSPHORYLATION REAGENT AND METHOD FOR DETECTING TARGET NUCLEIC ACID

摘要

The purpose of the present invention is to provide a method for evaluating the quality of a dephosphorylation reagent that contains alkaline phosphatase, and a method for detecting a target nucleic acid with the use of a dephosphorylation reagent that is evaluated as having a high quality by the aforesaid evaluation method. To achieve this purpose, provided is a method for evaluating the quality of a dephosphorylation reagent, said method comprising: step (1-1) for preparing a dephosphorylation reagent that contains alkaline phosphatase and a peptide fragment derived from the alkaline phosphatase; and step (1-2) for, when the ratio of the content of the peptide fragment to the content of the alkaline phosphatase is not more than a preset reference value, then evaluating the dephosphorylation reagent as having a high quality.