SNP Genetic Marker of Fat Deposition Traits in Pigs and Its Application

摘要

#$%^&*AU2020101763A420200917.pdf#####ABSTRACT The invention belongs to the technical field of livestock genetic marker preparation. In particular, the invention relates to a SNP genetic marker for porcine fat deposition traits and its application. A gene fragment of porcine fat deposition traits used as a genetic marker is obtained from porcine OLR1 gene. Its nucleotide sequence is shown in SEQ ID NO: 1 and FIG. 4. There is a A/G base mutation at 181 bp of the sequence shown in FIG. 4, which leads to Pag I-RFLP polymorphism. The invention also discloses a method for preparing a genetic marker of pig fat deposition traits and an application of the prepared genetic marker in detection of polymorphism of pig fat deposition traits, providing a new marker for markerassisted selection of pigs.-1/4BigWhite TCCTCGGTGGAAGAGCATTTGTTCGAGATGCAGAGTAGGTACCTGTTTTTTAGTCTGGCC 60 Landrace TCCTCGGTGGAAGAGCATTTGTTCGAGATGCAGAGTAGGTACCTGTTTTTTAGTCTGGCC 60 Qingping TCCTCGGTGGAAGAGCATTTGTTCGAGATGCAGAGTAGGTACCTGTTTTTTAGTCTGGCC 60 Meishan TCCTCGGTGGAAGAGCATTTGTTCGAGATGCAGAGTAGGTACCTGTTTTTTAGTCTGGCC 60 BigWhite TGGCCACTGATTACATGCATGTCCTTGGATGGACAGGGTATGGTGGGTCTCTAGGCTCCA 120 Landrace TGGCCACTGATTACATGCATGTCCTTGGATGGACAGGGTATGGTGGGTCTCTAGGCTCCA 120 Qingping TGGCCACTGATTACATGCATGTCCTTGGATGGACAGGGTATGGTGGGTCTCTAGGCTCCA 120 Meishan TGGCCACTGATTACATGCATGTCCTTGGATGGACAGGGTATGGTGGGTCTCTAGGCTCCA 120 Big White GCAGGCATGTCTATAAAACCATCCAGAAGATGGCAAAGATCCCTTTGGTTCTGTGGCTTC 180 Landrace GCAGGCATGTCTATAAAACCATCCAGAAGATGGCAAAGATCCCTTTGGTTCTGTGGCTTC 180 Qingping GCAGGCATGTCTATAAAACCATCCAGAAGATGGCAAAGATCCCTTTGGTTCTGTGGCTTC 180 Meishan GCAGGCATGTCTATAAAACCATCCAGAAGATGGCAAAGATCCCTTTGGTTCTGTGGCTTC 180 BigWhite GrGAATCTAATCTAAGATGTTCACATCAACTCTTTGATCAATTCAGAAATGTTCCTCCCT 240 Landrace GrGAATCTAATCTAAGATGTTCACATCAACTCTTTGATCAATTCAGAAATGTTCCTCCCT 240 Qingping ATGAATCTAATCTAAGATGTTCACATCAACTCTTTGATCAATTCAGAAATGTTCCTCCCT 240 Meishan ATGAATCTAATCTAAGATGTTCACATCAACTCTTTGATCAATTCAGAAATGTTCCTCCCT 240 Big White ACAGGAATTCATCCAGCAAACCATCGCCCATTCCAGTTTCCCATTCTGGATGGGGTTATC 300 Landrace ACAGGAATTCATCCAGCAAACCATCGCCCATTCCAGTTTCCCATTCTGGATGGGGTTATC 300 Qingping ACAGGAATTCATCCAGCAAACCATCGCCCATTCCAGTTTCCCATTCTGGATGGGGTTATC 300 Meishan ACAGGAATTCATCCAGCAAACCATCGCCCATTCCAGTTTCCCATTCTGGATGGGGTTATC 300 BigWhite TCTGAGGAAACCCAACAACTCATGGCTCTGGGAGGACGGTACTCCTTTGATGCCCCACTT 360 Landrace TCTGAGGAAACCCAACAACTCATGGCTCTGGGAGGACGGTACTCCTTTGATGCCCCACTT 360 Qingping TCTGAGGAAACCCAACAACTCATGGCTCTGGGAGGACGGTACTCCTTTGATGCCCCACTT 360 Meishan TCTGAGGAAACCCAACAACTCATGGCTCTGGGAGGACGGTACTCCTTTGATGCCCCACTT 360 Fig. 1A - continued in Fig. lB