DESIGN OF SEQUENCING PRIMERS AND PCR-BASED METHOD FOR SEQUENCING WHOLE GENOME

摘要

Disclosed are a sequencing primer set, comprising a universal upstream primer, a universal downstream primer, and a promoter-enriched downstream primer; and a PCR-based method for sequencing a whole genome, the method comprising: by using the universal upstream primer and the universal downstream primer, performing first PCR amplification on the DNA of a sample to be sequenced, so as to obtain a first amplification product, and by using the universal upstream primer and the universal downstream primer, performing second PCR amplification on the DNA of the sample to be sequenced, so as to obtain a second amplification product; and simultaneously performing sequencing on the two amplification products. According to the above-mentioned method, the genome size and the sample amount can be adjustable, and the requirements for DNA quality are not high; there is no need for known SNP markers and for pre-construction of chips; and the method can meet requirements for the detection and screening of the whole genome.