NEW PRIMER AND ANALYSIS METHOD OF SLA-3 GENOTYPE USING GENOMIC PCR-BASED DIRECT SEQUENCING

摘要

The present invention relates to an analysis method for SLA-3 genotype, and more specifically, to a method for analyzing SLA-3 genotype through direct sequencing of genomic DNA. According to the present invention, genotype of all SLA-3 alleles can be precisely and easily analyzed through PCR and direct sequencing based on genomic DNA without allele information and a cloning step, by designing a primer set which is suitable for genomic PCR, amplifies all alleles, and minimizes variability of the allele in amplification efficiency.;COPYRIGHT KIPO 2015