MASS SPECTROMETRIC METHODS AND COMPOSITIONS FOR IDENTIFYING MICROBES FROM BLOOD BY REDUCING CONFOUNDING PROTEINS

摘要

The direct identification of microorganisms from positive blood culture broths by protein analysis remains a challenge. Although positive blood culture broths are usually mono-bacterial or mono-fungal, the presence of proteins from red cells, white cells, and serum confounds analysis. Disclosed herein are unique compositions and methods for overcoming the challenges presented by confounding proteins in positive blood cultures. The disclosed compositions and methods are especially applicable to mass spectrometric based analyses for the identification of microorganisms in a blood culture.