METHOD FOR PRODUCING LAVA SEAWATER-DERIVED NATURAL MINERAL-COATED PROBIOTICS AND LAVA SEAWATER-DERIVED NATURAL MINERAL-COATED PROBIOTICS USING SAME

摘要

The present invention relates to a method for producing lava seawater-derived natural mineral-coated probiotics and lava seawater-derived natural mineral-coated probiotics using same. More specifically, the present invention uses a method for adding lava seawater to a medium for culturing lactobacilli so that the concentration of the lava seawater becomes 30-70% (v/v), thus causing minerals included in the lava seawater and proteins on the lactobacilli medium to precipitate in a mineral-protein salt state, thus inducing smooth culture of lactobacilli through the control of salinity on the medium, and while lactobacilli are cultured, chaperones, which are stress-responsive proteins, are produced in microbial bodies on a medium in which the concentration of salt is adjusted, due to salt-induced stress, and a culture broth after culture is concentrated so that the outer sides of the microbial bodies are uniformly coated with a mineral-protein salt generated when the medium is sterilized, and thus overall thermal stability of the inner and outer sides of the microbial bodies are remarkably enhanced. Through this, it is possible to obtain efficacy in remarkably enhancing the viability and storage stability over time when lactobacilli are lyophilized or spray-dried, efficacy in enduring continuous pH changes of gastric acid and bile acid in the digestive tract inside a body, and the like.