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3-KETOSE 3-EPIMERASE PRODUCED BY ARTHROBACTER GLOBIFORMIS

机译:节肢动物球菌产生的3-酮3-表异构酶

摘要

Challenge: Providing a method for producing highly stable epimerase and ketose for use in the food industry. Solution: ketose 3-epimerase obtained from microorganisms belonging to the genus Arthrobacter and having the amino acid sequence represented by SEQ ID NO: 1 in the sequence list and the following substrate specificity: (1) D- or L -Epimerize position 3 of ketose to generate the corresponding D- or L-ketose. (2) Among D- or L-ketose, substrate specificity for D-fructose and D-psicose is highest. And ketose 3-epimerase to generate a corresponding D- or L-ketose by epimerizing the position 3 of D- or L-ketose represented by SEQ ID NO: 3 or SEQ ID NO: 4 in the sequence list.
机译:挑战:提供一种生产高度稳定的差向异构酶和酮糖的方法,用于食品工业。解决方案:从节杆菌属的微生物中获得的酮糖3-差向异构酶,其氨基酸序列在序列表中由SEQ ID NO:1表示,并具有以下底物特异性:(1)酮糖的D-或L-表异构化位置3产生相应的D-或L-酮糖(2)在D-或L-酮糖中,对D-果糖和D-癸糖的底物特异性最高。酮糖3-表异构酶通过使序列表中SEQ ID NO:3或SEQ ID NO:4表示的D-或L-酮糖的位置3差向异构而产生相应的D-或L-酮糖。

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