A method for identifying pathogenic bacteria in food substrates using high throughput sequencing

摘要

FIELD: biotechnology.SUBSTANCE: invention relates to the field of biotechnology. Disclosed is a method for identifying pathogenic bacteria in food substrates using high-performance sequencing, comprising sampling biological material and recovering DNA with multiplex PCR, characterized by that for amplification in one reaction, 34 primers are simultaneously used: direct SEQ ID NO 1–17 and inverse SEQ ID NO 18–34, all amplified sequences are subjected to high-performance sequencing, method includes comparing the sequenced nucleotide sequences with sequences of bacteria Bacillus cereus, Campylobacter coli, Campylobacter jejuni, Clostridium perfringens, Clostridium tyrobutyricum, Cronobacter sakazii, Escherichia coli (EHEC), Escherichia coli (STEC/VTEC), Listeria monocytogenes, Mycobacterium tubercolosis, Proteus mirabilis, Salmonella enteric, Shigella flexneri, Shigella boydii, Shigella sonnei, Staphylococcus aureus, Vibrio cholera, Vibrio parahaemoliticus, wherein sequence identity over 95 % implies a presence of said pathogenic bacteria in the food substrate.EFFECT: invention can be used in food industry for identification of pathogenic bacteria both in incoming raw materials of food enterprises, and at different stages of production, including final products, including in laboratories for food products quality control, due to high efficiency.1 cl