TEST SYSTEM FOR IDENTIFICATION OF BEAR TISSUE (URSUS) IN DRY FODDER AND SEMI-FINISHED MEAT PRODUCTS

摘要

FIELD: biotechnology.;SUBSTANCE: invention relates to the field of biotechnology. Invention is a test system for identification of DNA of bear tissue (Ursus) in dry fodder and meat semi-products, comprising a buffer for carrying out a polymerase chain reaction, a mixture for carrying out thereof, consisting of deoxynucleoside triphosphates, oligonucleotide primers and fluorescent probes specific for the animal genome region and for the internal control sample; mixture of enzymes from DNA polymerase with enzyme inhibiting antibodies, TAQ POLYMERASE, an internal control sample in the form of a bacteriophage T4 suspension with concentration of 5×10³ phagic particles per 1 mcl, a negative control sample which is a mixture of recombinant plasmid DNA containing a fragment of the animal's genome and a fragment of the bacteriophage T4 genome.;EFFECT: invention can be used to expand functional capabilities and increase accuracy of identification of species identity, simplify sample preparation process.;1 cl, 5 tbl