METHOD FOR ASSESSING METASTATIC INVOLVEMENT OF SIGNALLING LYMPH NODE IN EARLY FORMS OF SQUAMOUS CELL CARCINOMA OF ORAL CAVITY

摘要

FIELD: medicine.;SUBSTANCE: invention relates to methods for pathological evaluation of metastatic involvement. Method for assessing the metastatic involvement of a signalling lymph node in early forms of squamous cell carcinoma of the oral cavity involves performing standard-step cuts of a signalling lymph node followed by immunohistochemical examination. Sections with thickness of 5 mcm are stained using hematoxylin and eosin, immunohistochemical examination is carried out using antibodies to Ki-67 and CD49 proteins, obtained coloured micro-preparations are scanned into high-resolution digital image, performing digital analysis of images using open source software, assessing the Ki-67 index as the number of positive-coloured nuclei per 1 square millimetre tissue lymph node, CD49 index is the number of positive-coloured membranes of tumour cells per 1 square millimetre tissue lymph node, cell density of tumour tissue is number of tumour cells per 1 square millimetre of tissue of lymph node and simultaneous combination of quantitative indices of index Ki-67, index CD49 and cell density of tumour tissue more than zero is estimated as presence of metastatic involvement of signal lymph node.;EFFECT: invention provides high reproducibility, which increases diagnostic accuracy.;1 cl, 1 tbl, 1 dwg