Method and microscope for recording a z-image stack from images of predetermined z-planes of an object

摘要

Method for recording a z-image stack from images of predetermined z-planes of an object with a microscope having a recording unit, the method comprising the following steps: a) Calculating a first total time for recording the z-image stack with a discrete recording mode, in which the Microscope approaches the predetermined z-planes one after the other and maintains its focus position of the approached z-plane during each acquisition of one of the images, b) calculating a second total time for the acquisition of the z-image stack with a continuous recording mode in which the microscope reaches the predetermined z-planes starts one after the other and during each recording one of the images continuously changes its focus position, andc) comparing the two total times and performing the recording mode, the total time calculated in steps a) and b) is shorter.