Method for determining a specificity of a real-time polymerase chain reaction with a melting curve analysis

摘要

The invention relates to a method (500) and a device (100) for determining the specificity of a real-time polymerase chain reaction, wherein a real-time polymerase chain reaction (501) is carried out, followed by a melting curve analysis (504) to determine the specificity , Before the start of the melting curve analysis (504) dyes, in particular fluorophores (221), the real-time polymerase chain reaction are deactivated.