METHOD FOR DETECTING MYCOBACTERIUM TUBERCULOSIS AND NONTUBERCULOUS MYCOBACTERIA USING DUPLEX REAL-TIME POLYMERASE CHAIN REACTION AND MELTING CURVE ANALYSIS

摘要

Disclosed are primer sets specific for the IS6110 gene characteristic of MTC and for the 16S rRNA gene characteristic of NTM, kits for the detection of MTC and NTM, comprising the same, and methods for detecting MTC and NTM by duplex real-time PCR and melting profile analysis using the same. Because the primers are exclusive to Mycobacterium tuberculosis and nontuberculous mycobacteria, the methods can be a clinical diagnostic means useful for the detection of both Mycobacterium tuberculosis and nontuberculous mycobacteria at the same time, with higher efficiency.