The present invention relates to a culture bag for culturing microorganisms, fungi, and other organisms, having a first wall and a second wall joined along side edges to form an inside for a bag containing an edible substrate and an organism to be cultured, At least one of the first wall and the second wall includes a bag made of a water vapor permeable layer through which water vapor passes, and a padding layer detachably coupled to the bag and made of a gas impermeable layer. The padding layer surrounds the water vapor permeable layer to prevent passage of the gas, and the padding layer has an opening with a gas filter patch, and the gas filter patch covers the opening to promote the cultivation of the organisms inside the bag. Is passed, and when the pad layer is removed from the bag, water vapor passes through the water vapor permeable layer to promote drying of the bag contents after the culture is completed.
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