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紫菜序列鉴定扩增区标记的建立方法

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摘要

本发明涉及种质资源，具体地说是一种紫菜序列鉴定扩增区标记的建立方法。它应用RAPD技术对紫菜材料进行遗传多样性分析，获得紫菜材料的特异性扩增片段；所述特异性片段经大量扩增后回收、克隆、测序得到紫菜材料P.tenuipedalis的RAPD扩增片段OPZ-19

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公开/公告号CN100408693C

专利类型发明授权
公开/公告日2008-08-06

原文格式PDF
申请/专利权人中国科学院遗传与发育生物学研究所;中国科学院海洋研究所;
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申请/专利号CN200310105267.9
发明设计人王斌;翁曼丽;金德敏;马家海;段德麟;
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申请日2003-12-05
分类号
代理机构沈阳科苑专利商标代理有限公司;
代理人许宗富
地址 100101 北京市安外大屯路917大楼
入库时间 2022-08-23 09:01:04

法律信息

法律状态公告日

法律状态信息

法律状态
2010-02-03

专利权的终止(未缴年费专利权终止)

专利权的终止(未缴年费专利权终止)
2008-08-06

授权

授权
2005-08-10

实质审查的生效

实质审查的生效
2005-06-08

公开

公开

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1. 紫菜序列鉴定扩增区标记的建立方法 [P] . 中国专利： CN100408693C . 2008.08.06
2. 扩增抗体重链前导序列及可变区基因序列的方法及其引物 [P] . 中国专利： CN101580831A . 2009-11-18
3. Method for screening indivu00ecduos of test to identify those having more probably best features of growth, development, reproduction and skeleton, such as rates of gain.The length of the skeleton or the size of the litter and to identify a genetic marker for growth rate of pig, the length of the skeleton, the size of the litter or infection of boar.Kit for assessing a DNA sample of suu00ecno, initiator to test how the presence of a place sph1 polu00ecmero gene CYP11A1 of suu00ecno, genetic marker associated with the rate of growth.The DNA sequence of the 5 'untranslated region of the gene CYP11A1 of suu00ecno, initiator designed to amplify a place of restriction sph1 polymorphic gene CYP11A1 of suu00ecno and method for screening pig. [P] . BR0010537A . 2002-04-16

机译：筛选测试个体的方法以鉴定那些可能具有生长，发育，繁殖和骨骼的最佳特征的动物，例如增重率。骨骼的长度或凋落物的大小，并鉴定生长的遗传标记猪的比率，骨骼的长度，猫砂的大小或公猪的感染。用于评估su u00ecno DNA样本的试剂盒，用于测试su u00eccmero基因sph1 pol u00ecmero基因CYP11A1如何存在的启动子su u00ecno基因CYP11A1的5'非翻译区的DNA序列，用于扩增su u00ecno限制性sph1多态性基因CYP11A1的位点的启动子和猪的筛选方法。
4. Nucleotide sequences of the mutant and non-mutant fragments of loci A and C of alleles of double low (00) fad3 form desaturase gene and low-linolenic mutant (LLMut) of oilseed rape plants (Brassica napus L. var. Oleifera), allospecific SNP markers specific for loci A and C of fad3 form desaturase gene - double low (00) and low-linolenic mutant (LLMut) of winter oilseed rape plants, nucleotide sequences of primer pairs for the amplification of loci A and C of fad3 form desaturase gene of winter oilseed rape plants, method for amplification of loci A and C of fad3 desaturase gene of winter oilseed rape plants, nucleotide sequences of the primers for the identification of mutant and non-mutant alleles of fad3 forms desaturase gene - double low (00) and low-linolenic mutant (LLMut) of oilseed rape in locus A and locus C in the microsequencing reaction, method for the identification of mutant and non-mutant alleles of fad3 form desaturase gene - double low (00) and low-linolenic mutant (LLM [P] . PL211126B1 . 2012-04-30

机译：双低（00）fad3等位基因的等位基因双低等位基因（A）的位点A和C的突变和非突变片段的核苷酸序列形成去饱和酶基因和油菜植物（甘蓝型油菜）的低亚麻酸突变体（LLMut），是同种异体对fad3基因座A和C特异的SNP标记形成去饱和酶基因-冬季油菜植物的双低（00）和低亚麻酸突变体（LLMut），用于扩增fad3基因去饱和酶A和C的引物对的核苷酸序列冬季油菜植物基因，fad3去饱和酶基因的基因座A和C的扩增方法，用于鉴定fad3形式脱氢酶基因的突变和非突变等位基因的引物的核苷酸序列-双低（00 ）和基因座A和基因座C中油菜的低亚麻酸突变体（LLMut）的微测序反应，fad3形式脱氢酶基因突变和非突变等位基因的鉴定方法-双低（00）和低亚麻酸突变体（法学硕士
5. Control primer annealing to improve the specificity of annealing in nucleic acid amplification, kit, methods for amplifying nucleic acid sequences of DNA and sequence of target nucleic acid or a mixture of nucleic acids, to detect the complementari Dade of DNA to mRNA differentially expressed in two or more samples of nucleic acid.To amplify a fragment of the cDNA target quickly, to amplify a population of cDNAs of filament double full-length complementary to mRNAs cDNAs, and filament double fortified with 5 'complementary to mRNAs to amplify rather than a sequence of nucleotide al VO at the same time, to produce a digital printing gDNA DNA, and RNA in a sample of mRNA.To identify segments of homology is conserved in a multigene family from a sample of mRNA, to identify a variation of nucleotide in a target nucleic acid, for mutagenesis in a target nucleic acid, and use of the initiator [P] . BRPI0214741B1 . 2015-05-12

机译：控制引物退火以改善核酸扩增中退火的特异性，试剂盒，扩增DNA核酸序列和靶核酸序列或核酸混合物的方法，检测DNA与mRNA互补表达的互补性（两种）或更多核酸样品。要快速扩增cDNA靶标的片段，以扩增与mRNA cDNA互补的长丝两倍全长的cDNA群体，并用与mRNA互补的5'双重修饰的长丝进行扩增而不是序列同时鉴定一个核苷酸的VO片段，以产生一个数字印刷的gDNA DNA和一个mRNA样品中的RNA。从一个mRNA样品中鉴定一个多基因家族中的同源片段是保守的，以鉴定一个核苷酸中一个核苷酸的变异。靶核酸，用于在靶核酸中诱变，以及引发剂的用途