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一种多肽抗原检测小鹅瘟抗体ELISA试剂盒

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摘要

一种多肽抗原检测小鹅瘟抗体ELISA试剂盒，属于生物技术检测领域，利用人工合成3种GPV‑VP3特异性偶联多肽抗原作为包被液快速检测GPV抗体，有效提高多肽与目的抗体的结合效率，从而显著提高检测灵敏度，同时显著降低非特异背景读值，特异性高。本发明的GPV抗体ELISA检测试剂盒具有操作简单、诊断速度快、成本低、在进行大规模检测中经济方便等优点,具有广泛的应用前景。

著录项

公开/公告号CN106680497B

专利类型发明专利
公开/公告日2018-07-10

原文格式PDF
申请/专利权人扬州大学;
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申请/专利号CN201611225705.9
发明设计人钱琨;顾晨曦;秦爱建;张杰;程晓薇;孔正茹;武宗仪;
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申请日2016-12-27
分类号
代理机构扬州市锦江专利事务所;
代理人江平
地址 225009 江苏省扬州市大学南路88号
入库时间 2022-08-23 10:13:44

法律信息

法律状态公告日

法律状态信息

法律状态
2018-07-10

授权

授权
2017-06-09

实质审查的生效 IPC(主分类):G01N33/569 申请日:20161227

实质审查的生效
2017-05-17

公开

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1. 一种多肽抗原检测小鹅瘟抗体ELISA试剂盒 [P] . 中国专利： CN106680497B . 2018.07.10
2. 一种检测小鹅瘟抗原的双抗体夹心ELISA试剂盒及其应用 [P] . 中国专利： CN104833801B . 2016.08.17
3. isolated polynucleotide, expression cassette, host cell, isolated polypeptide, non-human transgenic animal that has been altered to express an alkali resistant mutant polypeptide, an antibody that specifically binds to an alkali resistant mutant polypeptide, a mutation detection kit alk inhibitor resistance in a biological sample, and methods for evaluating a biological sample for an alk inhibitor resistance mutation to diagnose cancer that is resistant to or likely to develop resistance to at least one alkaline inhibitor. small alk molecule kinase, to evaluate a biological sample for an alk inhibitor resistance mutation, to diagnose cancer that is resistant to or likely to develop resistance to pf-02341066 in a patient, to specifically reduce expression of an alk-resistant mutant to treat cancer associated with activating aberrant loop capacity that is resistant to at least one small molecule alk kinase inhibitor treating an aberrant cancer that is resistant to pf-02341066, identifyin [P] . BR112012026239A2 . 2018-06-26

机译：分离的多核苷酸，表达盒，宿主细胞，分离的多肽，已被改变以表达耐碱突变体多肽的非人类转基因动物，与耐碱突变体多肽特异性结合的抗体，突变检测试剂盒生物样品，以及用于评估生物样品中是否存在对烷基抑制剂的抗性突变以诊断对至少一种碱性抑制剂有抗药性或可能发展出抗药性的癌症的方法。小分子激酶，用于评估生物学样品中是否具有抑制碱性磷酸酶抑制剂的突变，以诊断对患者具有抗药性或可能对pf-02341066产生抗药性的癌症，从而特异性降低抗癌突变体的表达以治疗癌症与激活抗至少一种小分子烷基激酶抑制剂的异常环路能力有关，该抑制剂治疗对pf-02341066有抵抗力的异常癌症
4. Polinucleotu00eddeo insulated tape of expression, host cell, isolated transgenic non-human animal polypeptide, which has been modified to express a polypeptide mutant with resistance to ALK, antibody that binds specifically to a polypeptide mutant with resistance to ALK, kit p For detection of a mutation with resistance to ALK inhibitor in a biological sample, and,Methods for evaluating a biological sample as a mutation with resistance to ALK inhibitor, for diagnosing a cancer that is resistant to or is likely to develop resistance to at least one inhibitor of kinase small molecule ALK, to evaluate a biological sample that Anto a mutation with resistance to ALK inhibitor.For diagnosing a cancer that is resistant to or is likely to develop resistance to at least one inhibitor of kinase small molecule ALK, to assess the biological sample as a mutation with resistance to ALK inhibitor, a cancer that is resistant to dianosticar Or is likely to develop resistance to PF - 02341066 in a pati [P] . BR112012026239A2 . 2016-11-29

机译：修饰的多聚核苷酸绝缘带，宿主细胞，分离的转基因非人类动物多肽，经过修饰以表达对ALK具有抗性的多肽突变体，与对ALK具有抗性的多肽突变体特异性结合的抗体，试剂盒p用于检测生物样品中对ALK抑制剂具有抗性的突变，以及用于评估生物样品为对ALK抑制剂具有抗性的突变的方法，用于诊断对至少一种对ALK抑制剂具有抗性或可能发展出抗性的癌症激酶小分子ALK，以评估Anto对ALK抑制剂具有抗性的突变的生物样品。用于诊断对至少一种激酶小分子ALK抑制剂具有抗药性或可能产生抗性的癌症，以评估该生物样品作为对ALK抑制剂有抗性的突变，即对狄诺司他抗性或可能对PF产生抗药性的癌症-02341066
5. methods for increasing and decreasing an effector function of a parental polypeptide and a parent antibody, for enhancing an effector function of a combination of at least one first and a second parent polypeptide, for inducing an effector response against a cell, cell membrane, or virion, to increase the specificity of a combination of at least a first and a second parent polypeptide, variant of a parent polypeptide, composition, and part kit [P] . BR112014000264A2 . 2017-02-14

机译：增加和减少亲本多肽和亲本抗体的效应子功能，增强至少一种第一和第二亲本多肽的组合的效应子功能，诱导针对细胞，细胞膜或病毒体的效应子反应的方法，以增加至少第一和第二亲本多肽，亲本多肽的变体，组合物和部分试剂盒的组合的特异性