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基于DNA条形码鉴定金钱白花蛇的特异引物及PCR法和试剂盒

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摘要

本发明属于中药及中药材鉴定技术领域，涉及一种基于DNA条形码的鉴定金钱白花蛇的特异性引物及PCR方法和试剂盒，用于对金钱白花蛇进行真伪鉴定，包括DNA提取、特异性PCR扩增、电泳检测等步骤，具有简便快捷、特异性高、通用性好等特点。本发明用于金钱白花蛇原动物及药材的鉴定，因其目标片段较短，特别适用于含金钱白花蛇的、且加工炮制过的药物制剂的快速鉴定。

著录项

公开/公告号CN104120182B

专利类型发明专利
公开/公告日2016-05-11

原文格式PDF
申请/专利权人南方医科大学;
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申请/专利号CN201410347863.6
发明设计人晁志;李晓蕾;曾伟萍;
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申请日2014-07-21
分类号C12Q1/68(20060101);C12N15/11(20060101);
代理机构31257 上海麦其知识产权代理事务所(普通合伙);
代理人董红曼
地址 510515 广东省广州市沙太南路1023号
入库时间 2022-08-23 09:39:35

法律信息

法律状态公告日

法律状态信息

法律状态
2018-08-03

未缴年费专利权终止 IPC(主分类):C12Q 1/68 授权公告日:20160511 终止日期:20170721 申请日:20140721

专利权的终止
2016-05-11

授权

授权
2016-05-11

授权

授权
2014-12-03

实质审查的生效 IPC(主分类):C12Q1/68 申请日:20140721

实质审查的生效
2014-12-03

实质审查的生效 IPC(主分类):C12Q 1/68 申请日:20140721

实质审查的生效
2014-10-29

公开

公开
2014-10-29

公开

公开

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1. 基于DNA条形码鉴定金钱白花蛇的特异引物及PCR法和试剂盒 [P] . 中国专利： CN104120182B . 2016.05.11
2. 基于DNA条形码的鉴别金钱白花蛇的引物及PCR-RFLP方法和试剂盒 [P] . 中国专利： CN104164492A . 2014-11-26
3. Nucleotide sequences of primers specific for the terminal areas of the tested DNA fragment conjugated with Rfo restorer gene for CMS ogura in rape, method for DNA amplification by polymerase chain reaction (PCR) with primers specific for genomic DNA fragment coupled with Rfo gene for CMS ogura in rape, process for the preparation of the nucleotide sequence of the DNA fragment conjugated the Rfo restorer gene for CMS ogura in rape, process for the preparation of SCAR type DNA marker for the identification of rape plants with Rfo restorer gene for ogura gene-cytoplasmic male sterility, method for the identification of rape plants with Rfo restorer gene for ogura gene-cytoplasmic male sterility and test kit for the identification of rape plants with Rfo restorer gene for ogura gene-cytoplasmic male sterility [P] . PL212433B1 . 2012-10-31

机译：油菜中与CMS ogura的Rfo恢复基因偶联的被测DNA片段末端区域特异的引物的核苷酸序列，通过基因组DNA片段的特异性引物与CMS ogura的Rfo基因偶联的聚合酶链反应（PCR）扩增DNA的方法在油菜中，制备与油菜CMS ogura的Rfo恢复子基因偶联的DNA片段的核苷酸序列的方法，在SCAR型DNA标记物的制备方法中用于鉴定含ogura基因的Rfo恢复子基因的油菜植物雄性不育，带有Rfo恢复基因的油菜植株的ogura基因胞质雄性不育的鉴定方法和试剂盒
4. Control primer annealing to improve the specificity of annealing in nucleic acid amplification, kit, methods for amplifying nucleic acid sequences of DNA and sequence of target nucleic acid or a mixture of nucleic acids, to detect the complementari Dade of DNA to mRNA differentially expressed in two or more samples of nucleic acid.To amplify a fragment of the cDNA target quickly, to amplify a population of cDNAs of filament double full-length complementary to mRNAs cDNAs, and filament double fortified with 5 'complementary to mRNAs to amplify rather than a sequence of nucleotide al VO at the same time, to produce a digital printing gDNA DNA, and RNA in a sample of mRNA.To identify segments of homology is conserved in a multigene family from a sample of mRNA, to identify a variation of nucleotide in a target nucleic acid, for mutagenesis in a target nucleic acid, and use of the initiator [P] . BRPI0214741B1 . 2015-05-12

机译：控制引物退火以改善核酸扩增中退火的特异性，试剂盒，扩增DNA核酸序列和靶核酸序列或核酸混合物的方法，检测DNA与mRNA互补表达的互补性（两种）或更多核酸样品。要快速扩增cDNA靶标的片段，以扩增与mRNA cDNA互补的长丝两倍全长的cDNA群体，并用与mRNA互补的5'双重修饰的长丝进行扩增而不是序列同时鉴定一个核苷酸的VO片段，以产生一个数字印刷的gDNA DNA和一个mRNA样品中的RNA。从一个mRNA样品中鉴定一个多基因家族中的同源片段是保守的，以鉴定一个核苷酸中一个核苷酸的变异。靶核酸，用于在靶核酸中诱变，以及引发剂的用途
5. Control primer annealing to improve the specificity of annealing in nucleic acid amplification kit.Methods for amplifying a nucleic acid sequence of a DNA or a mixture of nucleic acids.And for selectively amplifying a nucleic acid sequence of a target DNA or a mixture of nucleic acids, and a sequence of the target nucleic acid from a mRNA.Method for detecting the complementarity of DNA to mRNA differentially expressed in two or more samples of nucleic acid, methods for amplifying a fragment of the cDNA and DNA quickly target.And to amplify a population of cDNAs of filament double full-length complementary to mRNAs cDNAs, and filament double fortified with 5 'complementary to mRNAs,Method to amplify rather than a sequence of nucleotu00ecdeo target at the same time, methods for producing a digital printing gDNA DNA, and RNA in a sample of mRNA.Method for identifying segments of homology is conserved in a family of multigene from a sample of mRNA, a method for the mutagenesis in a target [P] . BR0214741A . 2004-11-23

机译：控制引物退火，以提高核酸扩增试剂盒中退火的特异性。扩增DNA或核酸混合物的核酸序列的方法以及选择性扩增靶DNA或核酸混合物的核酸序列的方法用于检测DNA与两个或多个核酸样品中差异表达的mRNA的互补性的方法，扩增cDNA和DNA片段的方法可快速靶向。全长与mRNA cDNA互补的双细丝cDNA的cDNA群体，以及与mRNA互补的5'增强双细丝的cDNA，同时扩增而不是核苷酸靶序列的方法，产生数字印刷gDNA DNA的方法mRNA样品中的RNA和RNA.mRNA样品的多基因家族中用于鉴定同源片段的方法是保守的，这是一种在靶标中诱变的方法