基于COI条形码序列的金钱白花蛇及其混伪品的DNA分子鉴定

摘要

目的:利用COI序列对金钱白花蛇及其常见混伪品进行DNA条形码鉴别,考察其可行性.方法:对金钱白花蛇及其混伪品共4个种11份样品的COI条形码序列进行研究,分析药材正品来源的种内变异,与混伪品的种间变异,以及系统树中物种的聚类情况.结果:银环蛇COI序列种内变异较小,最大K-2P距离为0.0185,与混伪品的种间序列差异较大,种间最小K-2P距离为0.1561,种间最小K-2P距离远大于种内最大K-2P距离.由所构建的系统聚类树可以看出,同属聚在一起,且各物种又形成相对独立的支,银环蛇COI序列可以明确地与混伪品区分开.结论:运用COI条形码序列能够准确鉴定金钱白花蛇的基源动物及其混伪品,为金钱白花蛇的鉴别提供了新的方法,在其他动物药基源物种鉴定中也具有较大的应用价值.%The CO1 barcode sequences have been utilized to distinguish Bungarus parvus from its adulterants. The COl barcode sequences of 11 samples from 4 species of Bungarus parvus and its adulterants were amplified and sequenced. The intra-specific variation and the inter-specific divergence were calculated and the cluster dendrogram was constructed. The results showed that the intra-specific sequence variation of Bungarus multicinctus, the origin animal of Bungarus parvus, exhibited low and the maximum intra-specific K-2P distance was 0.0185. The interspecific sequence divergence between B. multicinctus and its adulterants exhibited high. The minimum inter-specific K-2P distance was 0. 1561. The cluster dendrogram constructed can be seen that the same genus is together, and the different species separated well. B. multicinctus and adulterants can be distinguished obviously by COI sequences. It is concluded that Bungarus parvus and its adulterants can be correctly identified by using COI barcode sequence. This study offered a new tool to identify Bangarus parvus from its adulterants. The COI region is potentially to be used in the identification of other animal species in traditional Chinese medicine.