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trnH-psbA序列作为DNA条形码在鉴定南湖菱中的应用及鉴定方法

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摘要

本发明公开了trnH-psbA序列作为DNA条形码在鉴定南湖菱中的应用及鉴定方法，所述鉴定方法包括以下步骤：利用PCR扩增待测样品的trnH-psbA序列并进行测序，如果trnH-psbA序列的278bp碱基为A、308bp碱基为T，则判定待测样品为南湖菱。与传统的形态学鉴定方法和DNA指纹标记相比，本发明利用trnH-psbA序列作为DNA条形码鉴定南湖菱，可通过对trnH-psbA序列进行PCR扩增和测序，直接获得鉴定结果，检测准确性高、可重复性高，鉴定时间短。

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公开/公告号CN104293935B

专利类型发明专利
公开/公告日2016-04-06

原文格式PDF
申请/专利权人浙江省嘉兴市农业科学研究院(所);
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申请/专利号CN201410509124.2
发明设计人李军;李白;高广春;黄嬛;
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申请日2014-09-28
分类号
代理机构杭州天勤知识产权代理有限公司;
代理人胡红娟
地址 314016 浙江省嘉兴市秀洲区王江泾镇双桥
入库时间 2022-08-23 09:38:12

法律信息

法律状态公告日

法律状态信息

法律状态
2016-04-06

授权

授权
2015-02-18

实质审查的生效 IPC(主分类):C12Q 1/68 申请日:20140928

实质审查的生效
2015-01-21

公开

公开

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