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植物开花相关基因LFY/FLO同源片段的快速克隆方法及其专用引物

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摘要

本发明是植物开花相关基因

著录项

公开/公告号CN102268429B

专利类型发明专利
公开/公告日2013-01-30

原文格式PDF
申请/专利权人西南林业大学;
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申请/专利号CN201110217275.7
发明设计人鄢波;于丽霞;汤晓倩;姚有林;樊国盛;
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申请日2011-08-01
分类号
代理机构昆明合众智信知识产权事务所;
代理人康珉
地址 650204 云南省昆明市白龙寺
入库时间 2022-08-23 09:12:57

法律信息

法律状态公告日

法律状态信息

法律状态
2014-10-01

未缴年费专利权终止 IPC(主分类):C12N 15/11 授权公告日:20130130 终止日期:20130801 申请日:20110801

专利权的终止
2013-01-30

授权

授权
2012-01-25

实质审查的生效 IPC(主分类):C12N 15/11 申请日:20110801

实质审查的生效
2011-12-07

公开

公开

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1. 植物开花相关基因LFY/FLO同源片段的快速克隆方法及其专用引物 [P] . 中国专利： CN102268429B . 2013.01.30
2. 一种克隆梅花中LFY同源基因编码区全序列的方法 [P] . 中国专利： CN103667313B . 2016.06.29
3. Control primer annealing to improve the specificity of annealing in nucleic acid amplification, kit, methods for amplifying nucleic acid sequences of DNA and sequence of target nucleic acid or a mixture of nucleic acids, to detect the complementari Dade of DNA to mRNA differentially expressed in two or more samples of nucleic acid.To amplify a fragment of the cDNA target quickly, to amplify a population of cDNAs of filament double full-length complementary to mRNAs cDNAs, and filament double fortified with 5 'complementary to mRNAs to amplify rather than a sequence of nucleotide al VO at the same time, to produce a digital printing gDNA DNA, and RNA in a sample of mRNA.To identify segments of homology is conserved in a multigene family from a sample of mRNA, to identify a variation of nucleotide in a target nucleic acid, for mutagenesis in a target nucleic acid, and use of the initiator [P] . BRPI0214741B1 . 2015-05-12

机译：控制引物退火以改善核酸扩增中退火的特异性，试剂盒，扩增DNA核酸序列和靶核酸序列或核酸混合物的方法，检测DNA与mRNA互补表达的互补性（两种）或更多核酸样品。要快速扩增cDNA靶标的片段，以扩增与mRNA cDNA互补的长丝两倍全长的cDNA群体，并用与mRNA互补的5'双重修饰的长丝进行扩增而不是序列同时鉴定一个核苷酸的VO片段，以产生一个数字印刷的gDNA DNA和一个mRNA样品中的RNA。从一个mRNA样品中鉴定一个多基因家族中的同源片段是保守的，以鉴定一个核苷酸中一个核苷酸的变异。靶核酸，用于在靶核酸中诱变，以及引发剂的用途
4. Control primer annealing to improve the specificity of annealing in nucleic acid amplification kit.Methods for amplifying a nucleic acid sequence of a DNA or a mixture of nucleic acids.And for selectively amplifying a nucleic acid sequence of a target DNA or a mixture of nucleic acids, and a sequence of the target nucleic acid from a mRNA.Method for detecting the complementarity of DNA to mRNA differentially expressed in two or more samples of nucleic acid, methods for amplifying a fragment of the cDNA and DNA quickly target.And to amplify a population of cDNAs of filament double full-length complementary to mRNAs cDNAs, and filament double fortified with 5 'complementary to mRNAs,Method to amplify rather than a sequence of nucleotu00ecdeo target at the same time, methods for producing a digital printing gDNA DNA, and RNA in a sample of mRNA.Method for identifying segments of homology is conserved in a family of multigene from a sample of mRNA, a method for the mutagenesis in a target [P] . BR0214741A . 2004-11-23

机译：控制引物退火，以提高核酸扩增试剂盒中退火的特异性。扩增DNA或核酸混合物的核酸序列的方法以及选择性扩增靶DNA或核酸混合物的核酸序列的方法用于检测DNA与两个或多个核酸样品中差异表达的mRNA的互补性的方法，扩增cDNA和DNA片段的方法可快速靶向。全长与mRNA cDNA互补的双细丝cDNA的cDNA群体，以及与mRNA互补的5'增强双细丝的cDNA，同时扩增而不是核苷酸靶序列的方法，产生数字印刷gDNA DNA的方法mRNA样品中的RNA和RNA.mRNA样品的多基因家族中用于鉴定同源片段的方法是保守的，这是一种在靶标中诱变的方法
5. Isolated polypeptide or fragment thereof or their variant or a derivative thereof, isolated nucleic acid sequence, process for obtaining nucleotide sequence homologues, expression vector, host cell, process for preparing a recombinant polypeptide, antibody or a fragment thereof, detection method of N. MENINGITIDIS, diagnostic method of N. MENINGITIDIS infection, use of the polypeptide, use of the nucleic acid sequence, use of oligonucleotide primers, use of the antibody, use of a pharmaceutically effective amount of the polypeptide and the antibody, a pharmaceutical preparation, prevention or treatment method of the N. MENINGITIDIS infection, identification method of the polypeptide immunoreactive fragment. [P] . CZ20002172A3 . 2000-11-15

机译：分离的多肽或其片段或其变体或衍生物，分离的核酸序列，获得核苷酸序列同源物的方法，表达载体，宿主细胞，重组多肽，抗体或其片段的制备方法，猪新孢子虫的检测方法，N。MENINGITIDIS感染的诊断方法，多肽的使用，核酸序列的使用，寡核苷酸引物的使用，抗体的使用，药学有效量的多肽和抗体的使用，药物制剂，预防或N.MENINGITIDIS感染的治疗方法，多肽免疫反应性片段的鉴定方法。