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万能DNA片段扩增技术及万能DNA片段扩增试剂盒

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摘要

本发明万能DNA片段扩增技术及万能DNA片段扩增试剂盒提供了一种扩增大小在一定范围内的具有3′羟基和5′磷酸基的任意DNA片段的技术方法和用以实现这种技术的试剂盒，国际专利分类号为C12Q1/68，由于本发明合成了四个万能PCR引物，并使这些引物与待扩增DNA片段单链相连接，获得了两端带有万能PCR引物接头的待扩增的DNA片段模板分子，从而直接利用万能PCR引物将待扩增的DNA片段高效，方便地进行扩增。

著录项

公开/公告号CN1133344A

专利类型发明专利
公开/公告日1996-10-16

原文格式PDF
申请/专利权人夏庆杰;
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申请/专利号CN95111312.7
发明设计人夏庆杰;
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申请日1995-04-12
分类号C12Q1/68;C12Q1/25;C12N15/11;
代理机构
代理人
地址 610044 四川省成都市华西医科大学医学遗传室
入库时间 2023-12-17 12:44:03

法律信息

法律状态公告日

法律状态信息

法律状态
1998-09-02

专利申请的视为撤回

专利申请的视为撤回
1996-10-16

公开

公开

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1. 万能DNA片段扩增技术及万能DNA片段扩增试剂盒 [P] . 中国专利： CN1133344A . 1996-10-16
2. 一种用于从生物材料中直接扩增DNA片段的方法及试剂盒 [P] . 中国专利： CN102321618A . 2012-01-18
3. Control primer annealing to improve the specificity of annealing in nucleic acid amplification kit.Methods for amplifying a nucleic acid sequence of a DNA or a mixture of nucleic acids.And for selectively amplifying a nucleic acid sequence of a target DNA or a mixture of nucleic acids, and a sequence of the target nucleic acid from a mRNA.Method for detecting the complementarity of DNA to mRNA differentially expressed in two or more samples of nucleic acid, methods for amplifying a fragment of the cDNA and DNA quickly target.And to amplify a population of cDNAs of filament double full-length complementary to mRNAs cDNAs, and filament double fortified with 5 'complementary to mRNAs,Method to amplify rather than a sequence of nucleotu00ecdeo target at the same time, methods for producing a digital printing gDNA DNA, and RNA in a sample of mRNA.Method for identifying segments of homology is conserved in a family of multigene from a sample of mRNA, a method for the mutagenesis in a target [P] . BR0214741A . 2004-11-23

机译：控制引物退火，以提高核酸扩增试剂盒中退火的特异性。扩增DNA或核酸混合物的核酸序列的方法以及选择性扩增靶DNA或核酸混合物的核酸序列的方法用于检测DNA与两个或多个核酸样品中差异表达的mRNA的互补性的方法，扩增cDNA和DNA片段的方法可快速靶向。全长与mRNA cDNA互补的双细丝cDNA的cDNA群体，以及与mRNA互补的5'增强双细丝的cDNA，同时扩增而不是核苷酸靶序列的方法，产生数字印刷gDNA DNA的方法mRNA样品中的RNA和RNA.mRNA样品的多基因家族中用于鉴定同源片段的方法是保守的，这是一种在靶标中诱变的方法
4. Nucleotide sequences of primers specific for the terminal areas of the tested DNA fragment conjugated with Rfo restorer gene for CMS ogura in rape, method for DNA amplification by polymerase chain reaction (PCR) with primers specific for genomic DNA fragment coupled with Rfo gene for CMS ogura in rape, process for the preparation of the nucleotide sequence of the DNA fragment conjugated the Rfo restorer gene for CMS ogura in rape, process for the preparation of SCAR type DNA marker for the identification of rape plants with Rfo restorer gene for ogura gene-cytoplasmic male sterility, method for the identification of rape plants with Rfo restorer gene for ogura gene-cytoplasmic male sterility and test kit for the identification of rape plants with Rfo restorer gene for ogura gene-cytoplasmic male sterility [P] . PL212433B1 . 2012-10-31

机译：油菜中与CMS ogura的Rfo恢复基因偶联的被测DNA片段末端区域特异的引物的核苷酸序列，通过基因组DNA片段的特异性引物与CMS ogura的Rfo基因偶联的聚合酶链反应（PCR）扩增DNA的方法在油菜中，制备与油菜CMS ogura的Rfo恢复子基因偶联的DNA片段的核苷酸序列的方法，在SCAR型DNA标记物的制备方法中用于鉴定含ogura基因的Rfo恢复子基因的油菜植物雄性不育，带有Rfo恢复基因的油菜植株的ogura基因胞质雄性不育的鉴定方法和试剂盒
5. DNA FRAGMENTS, PRIMERS AND METHOD FOR AMPLIFICATION OF THE DNA FRAGMENTS AND KIT INCLUDING THE AFOREMENTIONED PRIMERS FOR THE DETECTION AND IDENTIFICATION OF CLINICALLY RELEVANT CANDIDA SPECIES [P] . 欧洲知识产权局专利： EP1888745A2 . 2008-02-20

机译： DNA片段，起始物和扩增DNA片段及试剂盒的方法，包括检测和鉴定临床相关念珠菌属的穿孔起始物