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一种用于从生物材料中直接扩增DNA片段的方法及试剂盒

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摘要

本发明公开了一种用于从生物材料中直接扩增DNA片段的方法及试剂盒。本发明的试剂盒包括：裂解液a：50-70v％PEG200、20-40mM KOH，pH13.2-13.8；裂解液b：5wt％-10wt％ chelex-100；裂解液c：5wt％-10wt％ chelex-100，1wt％-2wt％ TritonX-100；中和液：pH6.8-7.5的190-210mmol/L Tris-HCl缓冲液，还可进一步包含2×Taq PCR混合液。本发明的试剂盒及方法适用于细菌、动物组织、植物组织、真菌、血液、唾液和毛发等多种样品。

著录项

公开/公告号CN102321618A

专利类型发明专利
公开/公告日2012-01-18

原文格式PDF
申请/专利权人生工生物工程(上海)有限公司;
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申请/专利号CN201110295395.9
发明设计人芦丽亚;张丽娟;李威;
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申请日2011-09-30
分类号C12N15/10(20060101);
代理机构31259 上海脱颖律师事务所;
代理人李强
地址 201611 上海市松江区香闵路698号
入库时间 2023-12-18 04:17:16

法律信息

法律状态公告日

法律状态信息

法律状态
2014-02-05

发明专利申请公布后的视为撤回 IPC(主分类):C12N15/10 申请公布日:20120118 申请日:20110930

发明专利申请公布后的视为撤回
2012-10-17

实质审查的生效 IPC(主分类):C12N15/10 申请日:20110930

实质审查的生效
2012-09-19

著录事项变更 IPC(主分类):C12N15/10 变更前: 变更后: 申请日:20110930

著录事项变更
2012-01-18

公开

公开

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1. 一种用于从生物材料中直接扩增DNA片段的方法及试剂盒 [P] . 中国专利： CN102321618A . 2012-01-18
2. 一种用于口腔脱落细胞直接PCR扩增的预处理方法及试剂盒 [P] . 中国专利： CN113171136A . 2021-07-27
3. Control primer annealing to improve the specificity of annealing in nucleic acid amplification kit.Methods for amplifying a nucleic acid sequence of a DNA or a mixture of nucleic acids.And for selectively amplifying a nucleic acid sequence of a target DNA or a mixture of nucleic acids, and a sequence of the target nucleic acid from a mRNA.Method for detecting the complementarity of DNA to mRNA differentially expressed in two or more samples of nucleic acid, methods for amplifying a fragment of the cDNA and DNA quickly target.And to amplify a population of cDNAs of filament double full-length complementary to mRNAs cDNAs, and filament double fortified with 5 'complementary to mRNAs,Method to amplify rather than a sequence of nucleotu00ecdeo target at the same time, methods for producing a digital printing gDNA DNA, and RNA in a sample of mRNA.Method for identifying segments of homology is conserved in a family of multigene from a sample of mRNA, a method for the mutagenesis in a target [P] . BR0214741A . 2004-11-23

机译：控制引物退火，以提高核酸扩增试剂盒中退火的特异性。扩增DNA或核酸混合物的核酸序列的方法以及选择性扩增靶DNA或核酸混合物的核酸序列的方法用于检测DNA与两个或多个核酸样品中差异表达的mRNA的互补性的方法，扩增cDNA和DNA片段的方法可快速靶向。全长与mRNA cDNA互补的双细丝cDNA的cDNA群体，以及与mRNA互补的5'增强双细丝的cDNA，同时扩增而不是核苷酸靶序列的方法，产生数字印刷gDNA DNA的方法mRNA样品中的RNA和RNA.mRNA样品的多基因家族中用于鉴定同源片段的方法是保守的，这是一种在靶标中诱变的方法
4. Nucleotide sequences of primers specific for the terminal areas of the tested DNA fragment conjugated with Rfo restorer gene for CMS ogura in rape, method for DNA amplification by polymerase chain reaction (PCR) with primers specific for genomic DNA fragment coupled with Rfo gene for CMS ogura in rape, process for the preparation of the nucleotide sequence of the DNA fragment conjugated the Rfo restorer gene for CMS ogura in rape, process for the preparation of SCAR type DNA marker for the identification of rape plants with Rfo restorer gene for ogura gene-cytoplasmic male sterility, method for the identification of rape plants with Rfo restorer gene for ogura gene-cytoplasmic male sterility and test kit for the identification of rape plants with Rfo restorer gene for ogura gene-cytoplasmic male sterility [P] . PL212433B1 . 2012-10-31

机译：油菜中与CMS ogura的Rfo恢复基因偶联的被测DNA片段末端区域特异的引物的核苷酸序列，通过基因组DNA片段的特异性引物与CMS ogura的Rfo基因偶联的聚合酶链反应（PCR）扩增DNA的方法在油菜中，制备与油菜CMS ogura的Rfo恢复子基因偶联的DNA片段的核苷酸序列的方法，在SCAR型DNA标记物的制备方法中用于鉴定含ogura基因的Rfo恢复子基因的油菜植物雄性不育，带有Rfo恢复基因的油菜植株的ogura基因胞质雄性不育的鉴定方法和试剂盒
5. Method for identifying the site of insertion of a DNA segment whose sequence is known, inserted in a stretch of DNA genu00c8mico ignored the site of a body.Method for determining the sequence of a stretch of DNA genu00c8mico unknown of a body in the vicinity of a stretch known,The preparation to identify the site of insertion of a DNA segment whose sequence is known, inserted in an unknown place in genu00c8mico DNA of an organism.Kit to identify the site of insertion of a DNA segment whose sequence is known, inserted in an unknown place in the DNA genu00c8mico a body and use of said preparation. [P] . BR0300337A . 2004-11-03

机译：鉴定已知序列的DNA片段的插入位点的方法，插入一段DNA gen的序列中的方法忽略了身体的位置。确定一段未知的DNA gen的序列的方法一种已知序列的制备方法，用于鉴定序列已知的DNA片段的插入位点的制剂，插入到生物体基因组DNA的未知位置。试剂盒用于鉴定其DNA片段的插入位点该序列是已知的，插入人体的DNA基因的未知位置，并使用所述制剂。