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Adhesion of human gingival fibroblasts/Streptococcus mitis co-culture on the nanocomposite system Chitlac-nAg

机译:人牙龈成纤维细胞/微生物链球菌在纳米复合体系Chitlac-nAg上的粘附性

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摘要

Composite materials are increasingly used as dental restoration. In the field of biomaterials, infections remain the main reason of dental devices failure. Silver, in the form of nanoparticles (AgNPs), ions and salt, well known for its antimicrobial properties, is used in several medical applications in order to avoid bacterial infection. To reduce both bacterial adhesion to dental devices and cytotoxicity against eukaryotic cells, we coated BisGMA/TEGDMA methacrylic thermosets with a new material, Chitlac-nAg, formed by stabilized AgNPs with a polyelectrolyte solution containing Chitlac. Here we analyzed the proliferative and adhesive ability of human gingival fibroblasts (HGFs) on BisGMA/TEGDMA thermosets uncoated and coated with AgNPs in a coculture model system with Streptococcus mitis. After 48 h, HGFs well adhered onto both surfaces, while S. mitis cytotoxic response was higher in the presence of AgNPs coated thermosets. After 24 h thermosets coated with Chitlac as well as those coated with Chitlac-nAg exerted a minimal cytotoxic effect on HGFs, while after 48 h LDH release raised up to 20 %. Moreover the presence of S. mitis reduced this release mainly when HGFs adhered to Chitlac-nAg coated thermosets. The reduced secretion of collagen type I was significant in the presence of both surfaces with the co-culture system even more when saliva is added. Integrin β1 localized closely to cell membranes onto Chitlac-nAg thermosets and PKCα translocated into nuclei. These data confirm that Chitlac-nAg have a promising utilization in the field of restorative dentistry exerting their antimicrobial activity due to AgNPs without cytotoxicity for eukaryotic cells.
机译:复合材料越来越多地用作牙齿修复。在生物材料领域,感染仍然是牙科器械故障的主要原因。以其抗菌特性而闻名的纳米颗粒(AgNPs),离子和盐形式的银被用于多种医学应用中,以避免细菌感染。为了减少细菌粘附到牙科设备上以及减少对真核细胞的细胞毒性,我们在BisGMA / TEGDMA甲基丙烯酸热固性材料上涂覆了新材料Chitlac-nAg,该材料由稳定的AgNP与含有Chitlac的聚电解质溶液形成。在这里,我们分析了人牙龈成纤维细胞(HGF)在BisGMA / TEGDMA热固性塑料上未涂覆和涂覆AgNPs的增生和粘附能力,该模型在与链球菌共培养的共培养模型系统中。 48小时后,HGFs很好地粘附在两个表面上,而在有AgNPs涂层的热固性材料存在下,链球菌的细胞毒性反应更高。 24小时后,涂有Chitlac的热固性塑料和涂有Chitlac-nAg的热固性塑料对HGF的细胞毒性作用最小,而48小时后,LDH释放增加了20%。此外,主要是当HGF粘附到Chitlac-nAg包被的热固性材料上时,链球菌减少了这种释放。在共培养系统的两个表面均存在的情况下,添加唾液时,I型胶原蛋白分泌的减少更为显着。整合素β1紧密位于Chitlac-nAg热固性塑料的细胞膜上,而PKCα易位到细胞核中。这些数据证实,Chitlac-nAg在修复性牙科领域具有广阔的应用前景,归因于AgNP对真核细胞没有细胞毒性,因此发挥了抗菌活性。

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