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Stimulation of Interleukin-2 [IL-2] Release by Rhizophora mangle Bark Aqueous Extracts and Its Fractions

机译:Rhizophora mangle Bark水提取物及其组分对白细胞介素-2 [IL-2]释放的刺激作用

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摘要

Aims: The objective of the present study was to prepare fractions of polyphenols based on their ability to stimulate release of interleukin-2 (IL-2) from a human T-cell line, Jurkat, in the presence or absence of phorbol myristic acetate (PMA), and to identify the candidate components responsible for this activity.\udStudy Design: Rhizophora mangle L. [Rhizophoraceae] bark was collected in occident zone of CUBA, was boiled in distilled water and freezer dried for its fractionation.\udMethodology: The IL2-releasing the activity of different fractions were quantified using a BDOptEIA Human IL-2 ELISA kit using Jurkat Cells and PMA during the test. The ESI–MS fingerprints of the extracts [ESI-MS/MS] were acquired by the negative ion mode using a Micromass-Waters QTOF mass spectrometer.\udResults: Guided fractionations from Rhizophora mangle bark aqueous extracts in the evaluation of releasing activity of interleukin 2-stimulated and unstimulated with Jurkat T cells in the presence of PMA showed that the butanolic fraction had an interleukin 2 production of 250 pg mL-1 and an 89.9% yield of procyanidins. Mass spectral studies of the butanolic fraction reflected the presence of compounds that varied between 1000-1333 m/z, indicating the presence of procyanidins up to a tetramer polymerization level linked to glycosides based on monomeric units [epicatechin / catechin]. An increase of IL-2, without prior stimulation with PMA, in the Jurkat T cell model, had not been previously reported for the phenolic compounds.\udConclusion: The compounds characterized preliminarily confirmed the structural diversity of polyphenols present in Rhizophora mangle L plant and its capacity to stimulate release of IL 2.
机译:目的:本研究的目的是根据在存在或不存在佛波醇肉豆蔻酸乙酸酯的刺激下,从人T细胞系Jurkat刺激白细胞介素2(IL-2)释放的能力来制备多酚级分。 \ ud研究设计:将根瘤菌(Rhizophora mangle L. [Rhizophoraceae])的树皮收集在CUBA的欧美区,在蒸馏水中煮沸,然后冷冻干燥以进行分馏。在测试过程中,使用Jurkat Cells和PMA使用BDOptEIA人IL-2 ELISA试剂盒对释放不同组分的IL2进行定量。提取物的ESI-MS指纹图谱[ESI-MS / MS]使用Micromass-Waters QTOF质谱仪通过负离子模式获得。在PMA存在下用Jurkat T细胞进行2刺激和未刺激,结果表明丁醇级分的白细胞介素2产量为250 pg mL-1,原花青素的产率为89.9%。丁醇级分的质谱研究反映了在1000-1333 m / z之间变化的化合物的存在,表明存在高达四聚体聚合水平且与基于单体单元[epicatechin / catechin]的糖苷连接的原花青素。以前尚未在Jurkat T细胞模型中报道过在酚醛化合物中未事先用PMA刺激而增加IL-2的情况。\ ud结论:这些化合物的特征初步证实了在Rhizophora mangle L植物和其刺激IL释放的能力2。

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