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Enzymatic reaction of the immobilized enzyme on porous silicon studied by matrix-assisted laser desorption/ionization-time of flight-mass spectrometry

机译:Enzymatic reaction of the immobilized enzyme on porous silicon studied by matrix-assisted laser desorption/ionization-time of flight-mass spectrometry

摘要

Desorption/ionization on silicon mass spectrometry (DIOS-MS) is a matrix-free technique that allows for the direct desorption/ionization of low-molecular-weight compounds with little or no fragmentation of analytes. This technique has a relatively high tolerance for contaminants commonly found in biological samples. DIOS-MS has been applied to determine the activity of immobilized enzymes on the porous silicon surface. Enzyme activities were also monitored with the addition of a competitive inhibitor in the substrate solution. It is demonstrated that this method can be applied to the screening of enzyme inhibitors. Furthermore, a method for peptide mapping analysis by in situ digestion of proteins on the porous silicon surface modified by trypsin, combined with matrix-assisted laser desorption/ionization-time of flight-MS has been developed.
机译:硅质谱法(DIOS-MS)上的解吸/电离是一种无基质技术,可用于低分子量化合物的直接解吸/电离,而分析物几乎没有碎片。该技术对生物样品中常见的污染物具有相对较高的耐受性。 DIOS-MS已用于确定多孔硅表面上固定化酶的活性。还通过在底物溶液中添加竞争性抑制剂来监测酶的活性。证明了该方法可用于酶抑制剂的筛选。此外,已经开发了一种通过在胰蛋白酶修饰的多孔硅表面上原位消化蛋白质并结合基质辅助的激光解吸/电离飞行时间质谱技术进行肽图分析的方法。

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