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Theoretical study of the separation mechanism of ionizable compounds in capillary electrochromatography

机译:Theoretical study of the separation mechanism of ionizable compounds in capillary electrochromatography

摘要

The migration mechanism of ionizable compounds in capillary electrochromatography (CEC) is more complicated than in high performance liquid chromatography (HPLC) due to the involvement of electrophoresis and the second chemical equilibrium. The separation mechanism of ionizable compounds in CEC has been studied theoretically. The electrochromatographic capacity factors of ions (k *) in CEC and in the pressurized CEC are derived by phenomenological approach. The influence of pH, voltage, pressure on k* is discussed. in addition, the k * of weak acid and weak base are derived based on acid-base equilibrium and the influence of pH on k * is studied theoretically.
机译:由于涉及电泳和第二化学平衡,毛细管电色谱法(CEC)中可电离化合物的迁移机理比高效液相色谱法(HPLC)中的更为复杂。从理论上研究了CEC中可电离化合物的分离机理。通过现象学方法得出CEC和加压CEC中离子的电色谱容量因子(k *)。讨论了pH,电压,压力对k *的影响。另外,基于酸碱平衡推导了弱酸和弱碱的k *,并从理论上研究了pH对k *的影响。

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