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Microbial consortia from residential wastewater for bioremediation of phenol in a chemostat

机译:来自居民废水的微生物联盟,用于对化学恒温器中的苯酚进行生物修复

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摘要

The study aimed at evaluating the potential of employing microbial consortia from residential wastewater to degrade phenol, a common industrial pollutant. The consortia was subjected to high shock loads and different levels of phenol in continuous cultures. In the shock load study with 1000 ppm phenol in a 1.5 litre working volume chemostat, the culture with prior acclimatization to phenol demonstrated 17 times higher affinity towards phenol (i.e. Ks = 173 ppm). This resulted in double growth rate (µmax=Dc=0.04 h-1) compared to non-acclimatized culture. However, the levels of phenol reduction in both cases were almost identical, approximately 60%. This indicated that the performance of different types of bacteria present in the non-acclimatized culture which was comparable to the acclimatized culture although with less number of microbe types. The effects of two loading concentrations, namely 200 ppm and 1000 ppm were examined at constant dilution rate of 0.02 h-1. The feeding of 200 ppm exhibited 97% of phenol reduction in comparison to only 57% of 1000 ppm. This result reconfirmed the inhibitory effect of phenol reduction at concentration above 500 ppm as previously determined in batch culture. The study has successfully established key operating parameters and provided a useful guideline for future up-scaling studies and site trials.
机译:该研究旨在评估利用居民废水中的微生物聚生体降解常见工业污染物苯酚的潜力。该联盟在连续培养中承受了高冲击负荷和不同水平的苯酚。在1.5升工作容积的化学恒温器中使用1000 ppm苯酚进行的冲击负荷研究中,事先适应苯酚的培养物对苯酚的亲和力高17倍(即Ks = 173 ppm)。与未适应的文化相比,这导致双倍的增长率(µmax = Dc = 0.04 h-1)。但是,两种情况下苯酚的还原水平几乎相同,约为60%。这表明在非适应性培养物中存在的不同类型细菌的性能与适应性培养物相当,尽管微生物类型较少。在0.02 h-1的恒定稀释率下检查了两种装载浓度(即200 ppm和1000 ppm)的影响。进料为200 ppm时,苯酚减少了97%,而1000 ppm中只有57%。该结果再次证实了如先前在分批培养中所确定的,浓度高于500ppm时苯酚还原的抑制作用。该研究已成功建立了关键的运行参数,并为将来的扩展研究和现场试验提供了有用的指导。

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  • 作者

    Jusoh Norhani; Razali Firdausi;

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  • 年度 2008
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  • 原文格式 PDF
  • 正文语种 {"code":"en","name":"English","id":9}
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