首页> 外文OA文献 >Improved image analysis procedures for monitoring activated sludge systems with filamentous bulking
【2h】

Improved image analysis procedures for monitoring activated sludge systems with filamentous bulking

机译:改进的图像分析程序,用于监测带有丝状堆积的活性污泥系统

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务,本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文,但由于OA文献来源多样且变更频繁,仍可能出现获取不到、文献不完整或与标题不符等情况,如果获取不到我们将提供退款服务。请知悉。

摘要

Activated sludge systems are frequently used in wastewater treatment plant. This process is the most suitable and studied system. However, several problems are being always detected, such as filamentous bulking. Filamentous bulking is typically caused by an overabundance of filamentous organisms that interfere with the settling and compaction process. This phenomenon can be studied and related with settling parameters by automated image analysis using different microscopy acquisitions. However, by using these standard image analysis procedures some relevant information about the state of the sludge is enclosed. Conventional routines, using monochrome images are not suitable to detect the filamentous bacteria which are gram-positive or gram-negative. Moreover, the traditional image acquisition methodologies are not capable to detect both viable and damaged bacteria present within the sludge. Presently, the gram-stain evaluation is performed by visual inspection and manual counting using a microscope which is a tedious procedure. Also, to overcome the lack of viability information, an epifluorescence staining method composed with two nucleic acid-binding stains can be used. For this study, a lab-scale activated sludge reactor was monitored during 100 days through image analysis information and the operational parameters were modified inducing filamentous bulking. Morphological changes were investigated by using new acquisition methods such as epifluorescence staining LIVE/DEAD® BacLight™ Bacterial Viability Kit, the LIVE BacLight™ Bacterial Gram Stain Kit and the traditional bright field. The overall results revealed an improvement of the sludge morphological characterisation, combining these new image analysis procedures with the conventional routines.
机译:活性污泥系统经常用于废水处理厂。此过程是最合适和研究最多的系统。但是,总是会发现一些问题,例如丝状膨松。丝状膨松通常是由过多的丝状生物造成的,这些生物干扰沉降和压实过程。通过使用不同的显微镜采集进行自动图像分析,可以研究这种现象并将其与沉降参数相关联。但是,通过使用这些标准的图像分析程序,可以包含有关污泥状态的一些相关信息。使用单色图像的常规程序不适用于检测革兰氏阳性或革兰氏阴性的丝状细菌。此外,传统的图像采集方法无法检测污泥内存在的活细菌和受损细菌。目前,革兰氏染色评估是通过目视检查和使用显微镜的人工计数来进行的,这是一个繁琐的过程。另外,为了克服缺乏生存力信息的问题,可以使用由两种核酸结合染色剂组成的荧光染色方法。在本研究中,通过图像分析信息在100天内对实验室规模的活性污泥反应器进行了监控,并对运行参数进行了修改,从而引起丝状堆积。通过使用新的采集方法,例如表观荧光染色LIVE /DEAD®BacLight™细菌生存力试剂盒,LIVE BacLight™细菌革兰氏染色试剂盒和传统的明场,研究了形态学变化。总体结果表明,这些新的图像分析程序与常规程序相结合,改善了污泥的形态特征。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号