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Identification of candida clinical isolates by PCR fingerprinting : a contribution to the study of molecular epidemiology of candidiasis in portugal

机译:PCR指纹图谱鉴定念珠菌临床分离株:对葡萄牙念珠菌病分子流行病学研究的贡献

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摘要

PCR fingerprinting with a single non-specific primer (T3B) was used to type yeast clinical isolates obtained from two Medical Institutions in north Portugal. Of the 177 strains isolated, 112 were obtained from vaginal swabs, 24 from urine, 23 from the upper respiratory system, seven from the anal mucosa and 11 were isolated from various sources including blood, pus, catheter and peritoneal fluid. All the isolates belonged to the genus Candida being the profiles obtained highly similar within a species and clearly distinct among species. Seven different species were identified: C. albicans, C. tropicalis, C. glabrata, C. guilliermondii, C. parapsilosis, C. krusei and C.lusitaniae. This methodology also allowed the distinction of C. dubliniensis which is very closely related to C. albicans. Cluster analysis of the global fingerprints obtained originated, in general, groups corresponding to each species analysed except for five strains which were submitted to 26S and ITS rDNA sequencing to confirm their identity. We demonstrate that although C. albicans is the predominant species found, other Candida species were present accounting for over 20% of the strains isolated. The most common non albicans species were C. tropicalis, C. glabrata and C. parapsilosis. Due to its predominance over the other species C. albicans was present in all types of clinical material except in blood samples. C. tropicalis was mainly recovered from the urine and respiratory tract. This study represents the first large-scale approach to the knowledge of Candida species present in hospital settings in Portugal.
机译:使用具有单个非特异性引物(T3B)的PCR指纹图谱,对从葡萄牙北部两个医疗机构获得的酵母临床分离株进行分型。在分离出的177株菌株中,从阴道拭子中获得112株,从尿液中获得24株,从上呼吸系统获得23株,从肛门粘膜获得7株,从血液,脓液,导管和腹膜液等多种来源分离出11株。所有分离物均属于假丝酵母属,是在一个物种内获得高度相似且在物种之间明显不同的轮廓。确定了七个不同的物种:白色念珠菌,热带念珠菌,glabrata念珠菌,guilliermondii念珠菌,近缘念珠菌,克鲁斯念珠菌和lusitaniae。这种方法还可以区分出与白色念珠菌密切相关的杜比尼梭菌。通常,对获得的全球指纹图谱的聚类分析起源于与所分析的每个物种相对应的组,只有五个菌株经过了26S和ITS rDNA测序以确认其身份。我们证明虽然白色念珠菌是发现的主要种,但其他念珠菌种也存在,占分离菌株的20%以上。最常见的非白色念珠菌是热带念珠菌,光滑念珠菌和副念珠菌。由于白色念珠菌占优势,除血液样本外,所有类型的临床材料中均存在白色念珠菌。热带念珠菌主要从尿液和呼吸道中恢复。这项研究代表了了解葡萄牙医院环境中念珠菌物种的第一种大规模方法。

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