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The KLa influence on ethanol production by Pichia stipitis

机译:KLa对树干毕赤酵母产乙醇的影响

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摘要

Nowadays, there is a great interest in the development of technologies forethanol production as an alternative combustible, since it can be used insteadof petrol or to blend with petrol, reducing the country’s dependence on oiland imported fuel. It is known that the ethanol production by fermentationis influenced by several process conditions such as pH, temperature, mediumcomposition, oxygen availability, among others. Determining the most suitablefermentation conditions is of large importance for the establishment of asuccessful technology. In the present work, the influence of oxygen transfervolumetric rate (KLa) on xylose to ethanol bioconversion by the yeast Pichiastipitis NRRL Y-7124 was evaluated using a semi-defined fermentation mediumcontaining 90 g/l xylose. The assays were carried out in a bioreactor at 30°C,under different aeration conditions (0.5, 1.0, and 1.5 vvm) and stirring rates(200, 300 and 400 rpm) which resulted in KLa values of 2.3, 18.7 and 65.8 h-1respectively. According to the results, the bioconversion was dependenton the aeration rate employed, the highest ethanol production (27.1 g/l)being achieved when using a KLa of 2.3 h-1. The increase of this parameterto 18.7 and 65.8 h-1 promoted decreases of 52% and 100% on ethanolproduction, respectively. By using a KLa of 65.8 h-1 the ethanol production wastotally deviated to biomass production. Such results are of interest for thedevelopment of a suitable technology for ethanol production by Pichia stipitis.
机译:如今,作为替代可燃物的乙醇生产技术的发展引起了人们极大的兴趣,因为它可以代替汽油或与汽油混合使用,从而减少了该国对石油和进口燃料的依赖。众所周知,发酵产生的乙醇受到多种工艺条件的影响,例如pH,温度,培养基组成,氧的利用率等。确定最合适的发酵条件对于建立成功的技术至关重要。在目前的工作中,使用半定量发酵培养基(含90 g / l木糖)评估了氧气转运体积率(KLa)对木糖转化为酵母毕赤酵母NRRL Y-7124进行乙醇生物转化的影响。该测定法是在生物反应器中于30°C,不同曝气条件(0.5、1.0和1.5 vvm)和搅拌速度(200、300和400 rpm)下进行的,其KLa值分别为2.3、18.7和65.8 h- 1个。根据结果​​,生物转化取决于所采用的通气速率,当使用2.3 h-1的KLa时,可获得最高的乙醇产量(27.1 g / l)。该参数增加至18.7和65.8 h-1分别促进了乙醇生产的52%和100%的下降。通过使用65.8 h-1的KLa,乙醇的生产完全偏离了生物质的生产。这样的结果对于开发适用于由毕赤酵母产生乙醇的合适技术是有意义的。

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