Two dozen of bryophyte species was collected from native habitat types acrossudEurope to test if the ethanol extract can affect the growth of variousudmicroorganisms. After start-up test the ethanol extract did not show any influenceudon growth and development to Gram-negative bacteria and fungi Candida albicansud(ATCC10231). Thus, the further tests focused on the Gram-positive bactreia. Theudethanol extract of 5g bryophyte material was macerated in liquid nitrogen, andudthen extract was evaporated till dry. The dry residue was dissolved in 5ml ofudmethanol. This was used in further analyses by MIC and MBC techniques. Theudresult obtained showed the most effective extracts were those made from Pelliaudendiviifolia and Bazzania trilobata. Phytopathogenic bacteria and fungi C. albicansudshowed resistance to extracts of any bryophyte species tested. Gram-positiveudbacterias, namely Staphylococcus aureus (ATCC25923), Listeria monocytogenesud(ATCC19111) and Bacilus subtilis (ATCC6633) were intolerant to extracts ofudliverworts P. endiviifolia and B. trilobata. MIC value for two above mentionedudliverworts extract tested were 7-12mm, while referent antibiotic (rifampicin)udinhibitory zone were 15-35mm. The most resistant to any bryophyte extracts wasudEscherichia coli (ATCC25922), while the most sensitive was B. subtilis (ATCC6633)udwith MIC values obtained 0.01-0.19 mg/ml. The results obtained show clearudantimicrobial potential of P. endiviifolia and B. trilobata, however further researchudare needed.
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