首页> 外文OA文献 >Effect of Biocontrol Agent Pseudomonas fluorescens 2P24 on Soil Fungal Community in Cucumber Rhizosphere Using T-RFLP and DGGE
【2h】

Effect of Biocontrol Agent Pseudomonas fluorescens 2P24 on Soil Fungal Community in Cucumber Rhizosphere Using T-RFLP and DGGE

机译:T-RFLP和DGGE生物防治剂荧光假单胞菌2P24对黄瓜根际土壤真菌群落的影响

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务,本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文,但由于OA文献来源多样且变更频繁,仍可能出现获取不到、文献不完整或与标题不符等情况,如果获取不到我们将提供退款服务。请知悉。

摘要

Fungi and fungal community play important roles in the soil ecosystem, and the diversity of fungal community could act as natural antagonists of various plant pathogens. Biological control is a promising method to protect plants as chemical pesticides may cause environment pollution. Pseudomonas fluorescens 2P24 had strong inhibitory on Rastonia solanacearum, Fusarium oxysporum and Rhizoctonia solani, etc., and was isolated from the wheat rhizosphere take-all decline soils in Shandong province, China. However, its potential effect on soil fungal community was still unknown. In this study, the gfp-labeled P. fluorescens 2P24 was inoculated into cucumber rhizosphere, and the survival of 2P24 was monitored weekly. The amount decreased from 108 to 105 CFU/g dry soils. The effect of 2P24 on soil fungal community in cucumber rhizosphere was investigated using T-RFLP and DGGE. In T-RFLP analysis, principle component analysis showed that the soil fungal community was greatly influenced at first, digested with restriction enzyme Hinf I and Taq I. However, there was little difference as digested by different enzymes. DGGE results demonstrated that the soil fungal community was greatly shocked at the beginning, but it recovered slowly with the decline of P. fluorescens 2P24. Four weeks later, there was little difference between the treatment and control. Generally speaking, the effect of P. fluorescens 2P24 on soil fungal community in cucumber rhizosphere was just transient.
机译:真菌和真菌群落在土壤生态系统中起着重要作用,真菌群落的多样性可以作为各种植物病原体的天然拮抗剂。生物防治是保护植物的一种有前途的方法,因为化学农药可能会造成环境污染。荧光假单胞菌(Pseudomonas fluorescens)2P24对茄形毛细小球菌(Rastonia solanacearum),尖孢镰刀菌(Fusarium oxysporum)和茄状根瘤菌(Rhizoctonia solani)等具有较强的抑制作用,并从中国山东省的小麦根际通透性衰退土壤中分离得到。然而,其对土壤真菌群落的潜在影响仍然未知。在这项研究中,将gfp标记的荧光假单胞菌2P24接种到黄瓜根际中,并每周监测2P24的存活。数量从每克干土108降低到105 CFU。用T-RFLP和DGGE研究了2P24对黄瓜根际土壤真菌群落的影响。在T-RFLP分析中,主成分分析表明,土壤真菌群落首先受到限制酶Hinf I和Taq I的消化,但受不同酶消化的差异不大。 DGGE结果表明,土壤真菌群落在开始时受到了极大的冲击,但随着荧光假单胞菌2P24的下降而缓慢恢复。四周后,治疗和对照之间几乎没有差异。一般来说,荧光假单胞菌2P24对黄瓜根际土壤真菌群落的影响只是短暂的。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号