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Tyrosinase as a dual reporter gene for both photoacoustic and magnetic resonance imaging

机译:酪氨酸酶作为双报告基因,可用于光声和磁共振成像

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摘要

Reporter genes are useful scientific tools for analyzing promoter activity, transfection efficiency, and cell migration. The current study has validated the use of tyrosinase (involved in melanin production) as a dual reporter gene for magnetic resonance and photoacoustic imaging. MCF-7 cells expressing tyrosinase appear brown due to melanin. Magnetic resonance imaging of tyrosinase-expressing MCF-7 cells in 300 μL plastic tubes displayed a 34 to 40% reduction in T1 compared to normal MCF-7 cells when cells were incubated with 250 μM ferric citrate. Photoacoustic imaging of tyrosinase-expressing MCF-7 cells in 700 μm plastic tubes displayed a 20 to 57-fold increase in photoacoustic signal compared to normal MCF-7 cells. The photoacoustic signal from tyrosinase-expressing MCF-7 cells was significantly greater than blood at 650 nm, suggesting that tyrosinase-expressing cells can be differentiated from the vasculature with in vivo photoacoustic imaging. The imaging results suggest that tyrosinase is a useful reporter gene for both magnetic resonance and photoacoustic imaging.
机译:报告基因是用于分析启动子活性,转染效率和细胞迁移的有用的科学工具。目前的研究已证实酪氨酸酶(涉及黑色素的产生)作为磁共振和光声成像双重报告基因的用途。表达酪氨酸酶的MCF-7细胞由于黑色素而呈现褐色。当将细胞与250μM柠檬酸铁一起孵育时,在300μL塑料管中表达酪氨酸酶的MCF-7细胞的磁共振成像显示,与正常MCF-7细胞相比,T1降低了34%至40%。与正常的MCF-7细胞相比,在700μm塑料管中表达酪氨酸酶的MCF-7细胞的光声成像显示光声信号增加20到57倍。来自表达酪氨酸酶的MCF-7细胞的光声信号在650 nm处显着大于血液,这表明可以通过体内光声成像将表达酪氨酸酶的细胞与脉管系统区分开。成像结果表明酪氨酸酶是磁共振和光声成像的有用报告基因。

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